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Effects of Pinus massoniana bark extract on the size of HeLa cells via Nesprin-2 pathway

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Hypothesis of PMBE
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Submitted   Mar 20, 2020
Published   Jun 25, 2020
Issue    Vol 2 (2020)
DOI   10.25082/CCR.2020.01.003

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Xiaolu Zhang
1. Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China; 2. Department of gynecology and obstetrics, Jinshan Hospital of Fudan University, Shanghai 201508, China
Mengqi Li
Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China
Yingya Li
1. Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China; 2. Center of Reproductive Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 201204, China
Jiao Feng
1. Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China; 2. Department of Gastroenterology, Shanghai Tenth Peoples Hospital, Tongji University School of Medicine, Shanghai 200072, China
Yingyu Cui corresponding author
1. Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai 200092, China; 2. Key Laboratory of Arrhythmias of the Ministry of Education of China (Tongji University), Shanghai 200120, China; 3. Institute of Medical Genetics, Tongji University School of Medicine, Shanghai 200092, China

Abstract

Proanthocyanidins (PAs) is the main constituent of Pinus massoniana bark extract (PMBE). PMBE was reported to induce cell cycle arrest and apoptosis in HeLa cells. During cell division, cells synthesize protein in G1 and G2 phases and replicate chromatin in S phase during interphase, which increases cell mass. Nesprins, a kind of protein encoded by syne gene, is a vital part of cytoskeleton and plays a role in cell cycle progress and cell division. HeLa cells were used as a model to examine effects of PMBE on cell growth and Nesprins expression with MTT assay and RT- PCR analysis, respectively. The cell size was evaluated by counting the cell number in a fixed area under microscope. The results showed that the size of survival HeLa cells in PMBE-treated group was obviously larger than that of those in control group (p = 0.00223, < 0.01), while the mRNA expression level of Nesprin-2 decreased significantly in PMBE-treated group (p = 0.0201, < 0.05). On this account, we put forward a hypothesis that PMBE inhibits the expression of syne-2, which leads to the decrease of Nesprin-2 and further results in the size increase of HeLa cells.

Keywords
Proanthocyanidins (PAs), Pinus massoniana bark extract (PMBE), Cell size, HeLa cells, Nesprins

Article Details

Supporting Agencies
This project was funded by National University Students Innovation Training Program, grant number: 1500107070; Yangfan Project of Tongji University School of Medicine, grant number: 2012YF05; Fundamental Research Funds for the Central Universities, grant number: 22120180190 and Shanghai Educational Research Project grant number: C19154.
How to Cite
Zhang, X., Li, M., Li, Y., Feng, J., & Cui, Y. (2020). Effects of Pinus massoniana bark extract on the size of HeLa cells via Nesprin-2 pathway. Current Cancer Reports, 2(1), 41-47. https://doi.org/10.25082/CCR.2020.01.003
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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