Open Access Peer-reviewed Research Article

Phylogenetic analyses and genomic variation of the 2019-nCoV

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Phylogenetic tree of 2019-novel Coronavirus sequences
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Submitted   Jun 14, 2020
Published   Aug 24, 2020
Issue    Vol 2 No 1 (2020)
DOI   10.25082/JPBR.2020.01.004

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Faiz Ul Haq corresponding author
Department of Microbiology University of Health Sciences Lahore, Pakistan & Center for Biotechnology and Microbiology University of Swat, Pakistan
Sidrah Saleem
Department of Microbiology University of Health Sciences Lahore, Pakistan
Muhammad Imran
Department of Microbiology University of Health Sciences Lahore, Pakistan
Ayesha Ghazal
Department of Microbiology University of Health Sciences Lahore, Pakistan
Kashif Ahmad
Center for Biotechnology and Microbiology University of Swat, Pakistan & Department of Microbiology, Hazara University, Pakistan
Muhammad Roman
Department of Microbiology University of Health Sciences Lahore, Pakistan
Saeed Ur Rahman
Department of Nursing University of Health Sciences Lahore, Pakistan
Sami Ullah
Department of Forensic Science University of Health Sciences Lahore, Pakistan
Iftekhar Ahmad
Center for Biotechnology and Microbiology University of Swat, Pakistan
Habibah Mehmood
Department of Forensic Science University of Health Sciences Lahore, Pakistan
Wajahat Ullah
Center for Biotechnology and Microbiology University of Swat, Pakistan

Abstract

There is a rising global concern about the SARS CoV-2 as a public health threat. Complete genome sequence have been released by the worldwide scientific community for understanding the molecular characteristics and evolutionary origin of this virus. Aim of the current context is to present phylogenetic relationship and genomic variation of 2019-nCoV. Based on availability of genomic information, we constructed a phylogenetic tree including also representatives of other coronaviridae, such as Middle East respiratory syndrome, severe acute respiratory syndrome and Bat coronavirus. The phylogenetic tree analysis suggested that SARS CoV-2 significantly clustered with bat SARS like coronavirus genome, however structural analysis revealed mutation in Spike Glycoprotein and nucleocapsid protein. However our phylogenetic and genomic analysis suggests that bats can be the reservoir for this virus. Lack of forest might be the fact in association of bats with human environment. It is also difficult to study on bats due to absence of proper reagent and availability of few species for research. We confirm high sequence similarity (>99%) among sequenced SARS CoV-2 genomes, and 96% genome identity with the bat coronavirus, confirming the notion of a zoonotic origin of SARS CoV-2.

Keywords
SARS-CoV-2, coronavirus, CLUSTAL analysis, genomic variation

Article Details

How to Cite
Haq, F., Saleem, S., Imran, M., Ghazal, A., Ahmad, K., Roman, M., Rahman, S., Ullah, S., Ahmad, I., Mehmood, H., & Ullah, W. (2020). Phylogenetic analyses and genomic variation of the 2019-nCoV. Journal of Pharmaceutical and Biopharmaceutical Research, 2(1), 126-130. https://doi.org/10.25082/JPBR.2020.01.004
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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